Biosynthesis and antimicrobial activity of copper oxide nanoparticles

Mohamed, Mazhar D A; El-Deeb, Bahig A; Mohamed, Doaa A; Mosa, Mohamed A; Elsharouny, Tarek H M

doi:10.21608/jsasj.2023.334542

Biosynthesis and antimicrobial activity of copper oxide nanoparticles

Document Type : Research and Review Papers

Authors

¹ Agricultural Microbiology Department Faculty of Agriculture Sohag University Sohag 82524 Egypt

² Botany and Microbiology Department Faculty of Science Sohag University Sohag 82524 Egypt

³ Nanotechnology & Advanced NanoMaterials Laboratory (NANML) Plant Pathology Research Institute Agricultural Research Center Giza Egypt

10.21608/jsasj.2023.334542

Abstract

The development of simple, economical, and
effective technologies to produce nanoparticles is a global
priority. Microorganism-based biosynthesis methods
developed as an environmentally conscious, pristine, and
feasible alternative to conventional physical and chemical
approaches. This research outlines the synthesis of copper
oxide nanoparticles utilizing bacterial filtrated supernatant.
The investigation focused on variables such as pH, copper
concentration, and temperature. Optimal conditions for
nanoparticle formation were identified at a neutral pH, 4
mM copper concentration, and 35°C. Characterization of
the CuO NPs involved TEM, UV-Vis spectroscopy, XRD,
and FTIR to examine their pure form, revealing a maximum
particle size of 36.22 nm with spherical morphology.
Evaluating the antimicrobial efficacy on pathogenic bacteria
(Staphylococcus aureus and Pseudomonas aeruginosa) and
plant pathogenic fungi (Rhizoctonia solani and
Fusariumsolani) through the well diffusion method
affirmed the probable outcome of CuO-NPs as an efficient
antimicrobial agent, supporting and validating prior
assertions

Keywords