Isolation and characterization of actinophages specific to Streptomyces rochi from Sohag Governorate

Mohamed, Mazhar D A; Mankarious, Christeena O F; Hammad, Adel M M; Elsharouny, Tarek H M

doi:10.21608/jsasj.2023.334599

Isolation and characterization of actinophages specific to Streptomyces rochi from Sohag Governorate

Document Type : Research and Review Papers

Authors

¹ Agricultural Microbiology Department Faculty of Agriculture Sohag University Sohag 82524 Egypt

² Agricultural Microbiology Department Faculty of Agriculture Minia University Minia Egypt

10.21608/jsasj.2023.334599

Abstract

The objective of this study was to identify and separate
Streptomyces rochei-specific actinophages. From two
different sites of reclaimed soil in the Sohag Governorate
region, eight actinophage (streptomyces phage) isolates
specifically for Streptomyces rochei were successfully
identified. The samples were taken from reclaimed soil in
Alkawmel city and the New Sohag experimental farm of the
Faculty of Agriculture, Sohag University, in Sohag, Egypt.
All of the isolated phages (the actinophage) looked to be
head-and-tail types, according to electron micrographs of
the isolated phages. The eight isolates of the phage were
given the numbers 1, 2, 3, 4, 5, 6, 7, and 8. Each phage
isolate's heat inactivation point was investigated. Although
all phage isolates maintained their infectivity at 85 °C
during 10 minutes, they all demonstrated resistance to high
temperatures. It has been discovered that a pH of 7 is ideal
for phage infection, while phage isolates can survive in both
alkaline and acidic environments. The susceptibility of the
different phage isolates to UV light varies.

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